Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.
CFDA SE Cell Proliferation Dye is hydrolysed in cells to release green fluorescent carboxy-fluorescein, for detection in the FITC channel.
Lieferumfang: Each CFDA SE Cell Proliferation Assay kit contains ten single use (10×50 µg) dye vials, anhydrous DMSO for preparing dye stock solutions, and a detailed labelling protocol.
