Citrullinated Histone H3 (Clone 11D3) ELISA Kit
Lieferant: Cayman Chemical
An ELISA for quantifying Citrullinated Histone H3 in biological matrices.
- Detect CitH3 in cell culture supernatants and cell lysates
- Detect CitH3 from as few as 1,000 neutrophils in human and mouse
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure CitH3 levels down to 0,15 ng/ml
- Rapid assay; get results in under four hours
Histone H3 is citrullinated by PAD4 at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and NET formation. In vitro, CitH3 is released from neutrophils that have been stimulated with calcium ionophore during the process of NETosis, and can be freed from NETs by treatment with nuclease. The measurement of CitH3, either in cell lysates or released during NETosis can be used as a functional readout of pharmacological PAD inhibition.
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