DENARASE High Salt is an engineered version of the wild-type Serratia marcescens endonuclease, which is commonly used for DNA removal applications in bioprocessing. Compared to the wild-type DENARASE, a few amino acids were substituted to provide the enzyme with an improved salt tolerance without losing its specificity for nucleic acids.
- Designed for improved salt and pH tolerance: For highest process flexibility and efficiency
- Tailored to fit your process: Seamless integration into existing processes
- Compatible with DENARASE ELISA Kit: The one-for-all Serratia marcescens ELISA Kit
Like the wild-type S. marcescens endonuclease, DENARASE High Salt is capable of the non-specific cleavage of phosphodiester bonds between the nucleotide subunits of nucleic acids. The enzyme can hydrolyze all forms of DNA and RNA, such as single-stranded, double-stranded, linear, circular, or supercoiled.
The protein consists of two monomers with a calculated molecular weight of 27 kDa each. The calculated isoelectric point (pI) of DENARASE High Salt is at pH 7.83. Magnesium (Mg2+) is an essential co-factor and prerequisite for the nucleic acid clearance activity of the salt-tolerant endonuclease.
Similar to the wild-type DENARASE, the manufacturing process of DENARASE High Salt is based on recombinant expression in a Bacillus sp. production host. The gram-positive production strain is free of endotoxins. DENARASE High Salt is manufactured and supplied by c-LEcta GmbH.
DENARASE High Salt enables optimal and cost-effective removal of nucleic acids in biomanufacturing processes that benefit from higher salt concentrations. The enzyme is especially suitable for the use at bioprocessing-relevant 200-500 mM salt and pH 7.4-pH 8. It is designed to improve the efficiency of various applications:
Viral Vaccines
Viral Vectors for Cell and Gene Therapies
Viscosity Reduction in Lysates
Sample Preparation in Electrophoresis and Chromatography
Enzyme origin: Engineered from Serratia marcescens
Production host: Bacillus sp.
Temperature optimum: 37 °C
Magnesium optimum: 5 - 25 mM
Isoelectrical point: pH 7.83
Salt: 200 - 500 mM
Standards: Produced under ISO 9001 standard, without the use of antibiotics, Triton X-100 and raw materials of animal origin or TSE/BSE risk.
Lieferumfang: DENARASE High Salt is shipped by default in insulated boxes with cold packs as a precautionary measure to protect against unexpectedly elevated temperatures during transport. Storage at -20 °C ± 5 °C is recommended upon receipt of the product.
Verpackung: DENARASE High Salt is filled in non-pyrogenic, USP Class VI compliant vials. The product vials are shipped under qualified cooled conditions. Shipping temperature may differ from storage temperature without affecting product quality.
Bitte beachten To address concerns regarding DENARASE High Salt stability at elevated temperatures during transport, delays in shipping or custom clearances and/or refrigeration problems at the customer locations, the following stability tests were conducted:
Incubation of DENARASE High Salt
I) at 4 °C for 3 months
II) at room temperature for 28 days
III) at 30 °C for 3 days
