This medium is recommended for the detection of yeasts and molds in soft drinks and other beverages.
m-Green Yeast and Mold Broth is a relatively complex formulation compared to other media used for the detection of fungi and yeast. This allows excellent fungal growth. Bacterial growth is inhibited by an acidic pH. Peptones are an excellent source of nitrogen, carbon and amino acids. Yeast extract is the vitamin source. Dextrose serves as an energy source. Potassium phosphate is a buffering agent. Magnesium sulfate, thiamine, and diastase provide essential ions, minerals, and nutrients. Bromocresol green is the pH indicator, facilitating visualization of fungal growth in the broth. The colonies of yeasts are green due to the diffusion of bromocresol green into the colonies (alkaline reaction). Acidic end products from the colonies diffuse into themedium, further reducing the pH and causing the dye to turn yellow (acidic reaction).
Typical Composition: Peptone, Yeast extract, D(+)Glucose (Dextrose), Potassium dihydrogen phosphate, Magnesium sulfate anhydrous, Bromcresol green, Thiamine chloride hydrochloride, Copper(II) sulfate pentahydrate, Diastase, L(+)Tartaric acid (pH at 25 °C: 4,6 ± 0,2).
Pack stored upright, protected from light and properly sealed. After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed.
