TMB substrate kit, Pierce™
Lieferant: Thermo Fisher Scientific
The Pierce™ TMB Substrate Kit contains soluble TMB (3,3´,5,5´-tetramethylbenzidine) that is ideal for sensitive ELISA-based applications. TMB is a chromogenic substrate for horseradish peroxidase. Once oxidised by the enzyme, this substrate yields a blue product that absorbs at 370 nm and 652 nm. Adding sulfuric or phosphoric acid to stop the reaction changes the product to yellow that absorbs at 450 nm.
- • Available in traditional or ready-to-use formulations
- • No additional reagents or filtering required
- • Non carcinogenic
- • Various levels of sensitivity and development speed to suit any assay
- • No DMF or DMSO present in the reagents
Substrate type: HRP (Horseradish Peroxidase)
Detection method: Colorimeteric method
Lieferumfang: Kit contains TMB solution (0,4 g/L), 200 ml and peroxide solution, 200 ml.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...