4',6-Diamidin-2-phenylindol-dihydrochlorid (DAPI) Verwendung in der nuklearen Färbung

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40011.EA 174 EUR
40011.
4',6-Diamidin-2-phenylindol-dihydrochlorid (DAPI) Verwendung in der nuklearen Färbung
4',6-Diamidin-2-phenylindol-dihydrochlorid (DAPI)

DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate (catalog no. 40009), a more water soluble form of the dye.


  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • λEx/λEm (with DNA) = 358/461 nm
  • Off-white solid soluble in H2O
  • Store at 4°C and protect from light, especially in solution


DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Formel: C₁₆H₁₅N₅·2HCl
Molecular Weight: 350,25 g/mol
Lagertemperatur: Kühlschrank
MDL: MFCD00012681
CAS-Nummer: 28718-90-3

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