Iminobiotin agarose, Pierce™

Lieferant: Thermo Fisher Scientific
Pierce™
PIER20221EA 190 EUR
PIER20221
Iminobiotin agarose, Pierce™
Protein-Aufbereitungs Proteinreinigungs-Reagenzien
Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

Pierce™ Iminobiotin Agarose is effective in situations that require mild dissociation of the avidin-biotin complex. Normally, disrupting an avidin-biotin interaction requires 6-8 M guanidine-HCl, pH 1,5, an environment that is often too harsh for proteins to maintain native structure or activity. Iminobiotin binds at pH values above 9,5 and elution is achieved at pH 4,0. Relatively gentle elution buffers, such as 0,1 M acetic acid or 50 mM ammonium acetate buffer, pH 4,0 containing 500 mM NaCl can be used to recover bound proteins from Pierce™ Iminobiotin Agarose.
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