Pierce™ Iodination beads are Ø 3 mm polystyrene beads that are coated with an oxidising reagent, which provides efficient activation of 125I for protein or peptide iodination procedures.
- Derivatised, uniform, non porous polystyrene beads
- Iodine-125 incorporation as high as 99%; labeled protein recovery >90%
- Able to function over a broad pH and temperature range
- Iodinates in the presence of azides, detergents, urea and high salt
- Allows efficient iodination of cell membrane surface proteins
- Fast and easy to use – iodinations complete in 2 to 15 minutes
- Reaction stopped by simply removing beads from reaction mixture with tweezers or pasteur pipette; no reducing agent necessary to terminate reaction
The coated iodination beads provide for convenient and efficient iodine-labeling without the hassles and damaging oxidative effects normally associated with chloramine T and other solution-based methods. One or two beads can be added per milliliter of protein solution and then easily removed following the labeling reaction, thereby completely removing the oxidising reagent from the protein.
Iodination with Pierce™ Iodination Beads is compatible with many common buffer components. Detergents such as SDS, NP-40, and Triton® X-100, and denaturants such as urea, and high-salt concentrations (1,0 M NaCl) will not harm the reaction and may improve incorporation of the radioactive iodine by making tyrosines less hindered. Iodinations may be performed in phosphate, Tris, HEPES and other common buffers. The beads are not compatible with reducing agents.
