SYBR® Green II 10000× in DMSO RNA-Gel-Farbstoff, Sigma-Aldrich®
Lieferant: Merck
M-Clarity™ Qualitätsstufe = MQ100
SYBR® Green II is a highly sensitive stain.
- Orange solution
- 1 mL sufficient for 100 mini-gels
- Designing Safer Chemicals
- Suitable for postelectrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels
On denaturing agarose/formaldehyde gels and polyacrylamide/urea gels, the sensitivity of SYBR Green II RNA gel stain is reduced, though still superior to that of ethidium bromide. Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng RNA per band in agarose/formaldehyde gel or polyacrylamide/urea gel using 254 nm epi-illumination, and about 4 ng RNA per band using 300 nm trans-illumination. Staining agarose/ formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
SYBR is a registered trademark of Life Technologies
Lagertemperatur:
Gefrierschrank |
MDL:
MFCD00284716 CAS-Nummer: 172827-25-7 |
Spezifikation Testergebnisse
Optical density | 0,52 − 0,60 at 481 nm (±3 nm) |
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