Dithiothreitol (DTT, Cleland's Reagenz) ≥99,4% für die Biotechnologie
Lieferant: VWR Chemicals
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Achtung
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Synonyme:
DTT
, (R*,R*)-1,4-Dimercapto-2,3-butandiol
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Formel:
C₄H₁₀O₂S₂ Molecular Weight: 154,25 g/mol Siedepunkt: < 125 °C (1013 hPa) Schmelzpunkt: 42…44 °C Flammpunkt: >110 °C (offener Tiegel) Lagertemperatur: Kühlschrank |
MDL:
MFCD00004877 CAS-Nummer: 3483-12-3 EINECS: 222-468-7 |
Spezifikation Testergebnisse
Abs.@283nm (0.02M, Wasser) | ≤ 0,05 |
DNasen | Keine nachgewiesen |
Trocknungsverlust | < 0,5 % |
Schmelzpunkt | 39 - 43 °C |
Oxidierted DTT | < 0,5 % |
Proteasen | Keine nachgewiesen |
Reinheit (-SH Gehalt) | > 99,4 % |
RNasen | Keine nachgewiesen |
Löslichkeit (5%, Wasser) | Entspricht |
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