PIPES ≥99%, weißes Pulver für die Molekularbiologie
Lieferant: MP Biomedicals
Synonyme:
2,2'-(Piperazin-1,4-diyl)bis(ethansulfonsäure)
PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.
- Molecular Biology Reagent Grade
- Presentation: White Powder
- Effective buffering range: 6.1 - 7.5
- For sterilization it is recommended to filter sterilize using a 0.2 µm filter.
Formel:
C₈H₁₈N₂O₆S₂ Molecular Weight: 302,37 g/mol Siedepunkt: 499 °C (1013 hPa) Schmelzpunkt: > 300 °C (Lit.) Lagertemperatur: Raumtemperatur |
MDL:
MFCD00006159 CAS-Nummer: 5625-37-6 EINECS: 227-057-6 Merck Index: 12,07633 |
Spezifikation Testergebnisse
Identity Test | Passes |
Purity | ≥99% |
FTIR | Conforms to Standard |
UV/VIS: OD260 (0.1M in 0.2M NaOH) | ≤0.05 |
UV/VIS: OD280 (0.1M in 0.2M NaOH) | ≤0.05 |
Solubility (0.1M in 0.2N NaOH) | Clear and Complete |
pKa (25°C) | 6.6-7.0 |
Moisture (LOD) | ≤1.0% |
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