Pikovskayas agar is recommended for detection of phosphate-solubilizing soil microorganisms.
- White to light yellow homogeneous free flowing powder
- Firm, comparable with 1,5% agar gel
- Must be stored below 30 °C in tightly closed container
Phosphate exists in both organic as well as inorganic forms in soil. Organic matter derived from dead and decaying plant debris is rich in organic sources of phosphorus. However, plants are able to utilize phosphorus from soil only in the free available form. Soil phosphates are rendered available either by plant roots or by soil microorganisms. Therefore, phosphate-dissolving soil organisms play a part in correcting phosphorus deficiency of crop plants. Pikovskayas Agar was modified by Sundara Rao and Sinha for detection of phosphate-solubilizing bacteria from soil. Yeast extract in the medium provides nitrogen and other nutrients necessary to support bacterial growth. Dextrose acts as an energy source. Different salts and yeast extract supports the growth of organisms. Phosphate-solubilizing bacteria will grow on this medium and form a clear zone around the colony, formed due to phosphate solubilization in the vicinity of the colony.