Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.
- Formulation is optimised for the initial seeding of 4000 cells/cm², allowing growth up to confluence (approximately 90%)
- Use of feeder layers, matrix substrates, or additional substances is unnecessary
- Due to the possibility of reduced proliferative activity, it is recommended to use the antibiotic supplement for freshly isolated cells only
To address potential reductions in proliferative activity, we recommend utilizing the antibiotic supplement exclusively for freshly isolated cells. The smooth muscle cell growth medium undergoes comprehensive testing after each production. All components are subjected to rigorous biological assays.
Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morphology, adherence rate, colony-forming efficiency, and population doubling time.
Storge: Store the supplemented Smooth muscle cell growth medium in a dark environment at temperatures ranging from 4 to 8 °C for a maximum duration of one month. Avoid heating the medium beyond 37 °C and refrain from using uncontrolled heat sources, such as microwave appliances. When utilising a portion of the medium, extract the required amount from the bottle and warm it appropriately. Never freeze the medium, as this could result in elevated salt concentrations due to the separation of pure water during freezing, leading to irreversible damage.
