Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.
- Purity >90% by flow cytometry, Test negative for Hepatitis B and C, HIV 1/2, HTLV-I/II, HIV-1/HCV/HBV, Cryopreserved in CryoStor® CS10 media
These differentiated procedures ensure the precise isolation of the intended T-cell groups. CD4⁺ T cells play a role in coordinating immune responses, while CD8⁺ T cells exert a cytotoxic function.
Cells were collected from from a healthy human donors using permission forms and procedures that have been approved by the Institutional Review Board (IRB). Storage: For prolonged cell preservation or resuscitation, it is recommended to prepare the cells for extended storage in the vapor phase of liquid nitrogen.
Storage of cells in the liquid phase of nitrogen is strongly discouraged to prevent potential cross-contamination risks when removing from storage. Long-term storage under −175 °C and for short-term storage, about one or two weeks at −80 °C is permitted, but it is recommended to minimise this duration to ensure optimal stability. Thawed samples should be used immediately and not stored.
