Phenol : Chloroform : Isoamylalkohol (25:24:1) (v/v/v) ≥99%, Biotechnologische Qualität, niedriger pH

Lieferant: VWR Chemicals
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Synonyme: Carbolic acid, Hydroxybenzene, Phenic acid

0966-400MLEA 352 EUR
0966-400ML
Phenol : Chloroform : Isoamylalkohol (25:24:1) (v/v/v) ≥99%, Biotechnologische Qualität, niedriger pH
Phenol : Chloroform : Isoamylalkohol

Phenol / Chloroform #2- A 25:24:1 mixture of phenol, chloroform, and isoamyl alcohol useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA.


  • Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
  • Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
  • Optimized pH for the Purification of RNA
  • Long Term Stability > One Year


For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

Lagertemperatur: Raumtemperatur
MDL: MFCD00133763
CAS-Nummer: 136112-00-0
UN: 2810
ADR: 6.1,II

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Spezifikation Testergebnisse

O.D.@330nm ≤ 0,2
O.D.@405nm ≤ 0,1
O.D.@510nm ≤ 0,1
pH@25°C 4,9 - 5,5
Reinheit ≥ 99 %

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