4',6-Diamidin-2-phenylindol-dilactat (DAPI) fluoreszierender Farbstoff, Verwendung in der nuklearen Färbung

Lieferant: Biotium
Achtung

Synonyme: 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide dilactate, 2-(4-Amidinophenyl)indole-6-carboxamidine dilactate

40009
40009.EA 182 EUR
40009.
4',6-Diamidin-2-phenylindol-dilactat (DAPI) fluoreszierender Farbstoff, Verwendung in der nuklearen Färbung
4',6-Diamidin-2-phenylindol-dilactat (DAPI)

DAPI dilactate is essentially the same as DAPI dihydrochloride (catalog no. 40011), but with a different counterion. The dilactate salt form of the dye is more water soluble than the dihydrochloride salt, and therefore DAPI dilactate is a better choice for preparing stock solutions in water.


  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • λEx/λEm(DNA) = 358/461 nm
  • Yellow solid soluble in water
  • Store at 4°C and protect from light, especially in solution


DAPI is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.


DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Formel: C₁₆H₁₅N₅·₂C₃H₆O₃
Molecular Weight: 457,48 g/mol
Lagertemperatur: Kühlschrank
CAS-Nummer: 28718-90-3
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