4',6-Diamidin-2-phenylindol-dilactat (DAPI) fluoreszierender Farbstoff, Verwendung in der nuklearen Färbung
Lieferant: Biotium
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Achtung
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Synonyme:
2-(4-Carbamimidoylphenyl)indole-6-carboximidamide dilactate, 2-(4-Amidinophenyl)indole-6-carboxamidine dilactate
DAPI dilactate is essentially the same as DAPI dihydrochloride (catalog no. 40011), but with a different counterion. The dilactate salt form of the dye is more water soluble than the dihydrochloride salt, and therefore DAPI dilactate is a better choice for preparing stock solutions in water.
- Classic blue nuclear counterstain for fixed cells
- Can be used at higher concentrations to stain live cells
- λEx/λEm(DNA) = 358/461 nm
- Yellow solid soluble in water
- Store at 4°C and protect from light, especially in solution
DAPI is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.
DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.
Formel:
C₁₆H₁₅N₅·₂C₃H₆O₃ Molecular Weight: 457,48 g/mol Lagertemperatur: Kühlschrank |
CAS-Nummer:
28718-90-3 |
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