Ethidium homodimer I Verwendung in der nuklearen Färbung
Lieferant: Biotium
|
Gefahr
|
Synonyme:
EthD-1, EtDi
Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.
- λEx/λEm (with DNA) = 528/617 nm
- λAbs (in H2O, no DNA) = 493 nm
- Cell permeability: Membrane impermeant
- Colors: Red
- Red solid soluble in DMSO or MeOH
- Store at 4°C and protect from light
Biotium offers a high purity grade Ethidium Homodimer I that is not available from other manufacturers. Ethidium Homodimer from other suppliers often contains a high amount (as much as 20%) of inorganic salt that lowers the weight percent purity. We also offer Ethidium Homodimer I, 2 mM solution in DMSO (catalog no. 40014).
Also see Ethidium Homodimer III, which was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.
EthD-I is dead cell specific in all cell types, including mammalian cells, bacteria and yeast.
Formel:
C₄₆H₅₀Cl₄N₈ Molecular Weight: 856,75 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00210948 CAS-Nummer: 61926-22-5 UN: 3096 ADR: 8 (4.3),II |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...