bisbenzimide H-33342 trihydrochloride (Hoechst 33342) 10 mg/mL in Wasser
Lieferant: Biotium
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Gefahr
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Synonyme:
Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, 2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole, bisBenzimide
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258, but either dye can be used to stain live or fixed cell in buffer or medium at 1 ug/mL, with no wash step required.
- For ease of use and safer handling
- λEx/λEm (with DNA) = 350/461 nm
- Light yellow solution
- Store at 4°C and protect from light
Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.
Formel:
C₂₇H₂₈N₆O·3HCl Molecular Weight: 561,93 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00012679 CAS-Nummer: 23491-52-3 EINECS: 245-691-1 |
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