bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)

Lieferant: Biotium
Gefahr

Synonyme: Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, bisBenzimide

40047
40047.EA 323 EUR
40047.
bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)
bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains.


  • λEx/λEm = 350/461 nm (with DNA)
  • Pale yellow solid soluble in water up to 2% (w/v)
  • Store at 4°C and protect from light


Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O (catalog no. 40046).


Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Formel: C₂₇H₂₈N₆O·3HCl·3H₂O
Molecular Weight: 615,99 g/mol (561,94 anhy)
Lagertemperatur: Kühlschrank
MDL: MFCD00012679
CAS-Nummer: 23491-52-3
EINECS: 245-691-1

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