bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)
Lieferant: Biotium
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Gefahr
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Synonyme:
Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, bisBenzimide
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains.
- λEx/λEm = 350/461 nm (with DNA)
- Pale yellow solid soluble in water up to 2% (w/v)
- Store at 4°C and protect from light
Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O (catalog no. 40046).
Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.
Formel:
C₂₇H₂₈N₆O·3HCl·3H₂O Molecular Weight: 615,99 g/mol (561,94 anhy) Lagertemperatur: Kühlschrank |
MDL:
MFCD00012679 CAS-Nummer: 23491-52-3 EINECS: 245-691-1 |
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