FLUO 3/AM 1 mM in anhydrischem DMSO fluoreszierender Indikator, zur Kalziumbestimmung
Lieferant: Biotium
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.
- Absorption/Emission For Fluo-3: 506 nm/526 nm (low or high [Ca2+] )
- Extinction Coefficient For Fluo-3: 86,000 M -1 cm -1 (506 nm)
- Cell permeability: Membrane permeant
- Orange red solid soluble in DMSO
- Store -20°C and protect from light, especially when in solution
Formel:
C₅₁H₅₀Cl₂N₂O₂₃ Lagertemperatur: Gefrierschrank |
CAS-Nummer:
121714-22-5 |
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