Rhod-590 AM ester fluoreszierender Indikator, zur Kalziumbestimmung
Lieferant: Biotium
50025
50025.EA
637
EUR
50025.
Rhod-590 AM ester fluoreszierender Indikator, zur Kalziumbestimmung
Rhod-590 AM ester
Membrane-permeant form of Rhod-590 calcium indicator dye that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. Rhod-590 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Rhod-590 by endogenous esterases once it enters cells.
- Absorption/Emission 590nm/616nm (low [Ca2+] )
- Absorption/Emission 595nm/626nm (high [Ca2+] )
- Kd 0.61μM
- Cell permeability: Membrane permeant
- Dark purple solid soluble in DMSO
- Store at -20°C and protect from light, especially in solution
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