FURA-2 pentasodium salt ≥95% (durch HPLC) fluoreszierender Indikator, zur Kalziumbestimmung
Lieferant: Biotium
Fura-2, pentasodium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading.
- Kd: 145 nM (22°C in pH 7.2 buffer)
- λEx/λEm (no Ca2+) = 363/512 nm; λEx/λEm (high Ca2+) = 335/505 nm
- ε (363 nm, no Ca2+) = 27,000 M-1cm-1; ε (335 nm, high Ca2+) = 35,000 M-1cm-1
- Cell permeability: Membrane impermeant
- Light yellow solid soluble in DMSO
- Store at 4°C and protect from light, especially in solution
Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.
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Formel:
C₂₉H₂₂N₃Na₅O₁₄ Molecular Weight: 751,45 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00083328 CAS-Nummer: 96314-98-6 |
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