Furaptra (Mag-Fura-2) tetrasodium salt ≥95% (durch HPLC) fluoreszierender Indikator, zur Kalziumbestimmung
Lieferant: Biotium
Synonyme:
Mag-Fura-2 tetrasodium salt
Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2+ but with a significantly higher Kd than Fura-2 for Ca2+.
- Absorption/Emission 369nm/511nm (H2O)
- Extinction Coefficient 22,000
- Cell permeability: Membrane impermeant
- Colors: Green
- Light yellow solid soluble in water (pH > 6)
- Store at 4°C and protect from light
An important application of Mag-Fura-2 is its use in detecting high, transient Ca2+ concentration during Ca2+ spikes. Mag-Fura-2/tetrasodium salt can be loaded into cells by microinjection or scrape loading. Also see Mag-Fura-2, tetrapotassium salt (50035) and the cell membrane-permeant Mag-Fura-2 AM ester (50037, 50038, 50039).
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