Di-8-ANEPPS Membranfarbstoff, für die Histologie
Lieferant: Biotium
Synonyme:
4-(2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl)-1-(3-sulfopropyl)pyridinium inner salt
ANEPPS dyes are fast responding membrane potential dyes. Compared to Di-4-ANEPPS, Di-8-ANEPPS is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS
- λEx/λEm (MeOH) = 498/713 nm*
- Orange red solid soluble in DMF, ethanol or DMSO
- Store at 4°C and Protect from light, especially when in solution
Biotium offers both Di-4-ANEPPS and Di-8-ANEPPS. Di-4-ANEPPS has the problem of being rapidly internalized in cells, so it is mainly used for short-term experiments. However, because Di-8-ANEPPS is more lipophilic, the dye is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS.
* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.
Formel:
C₃₆H₅₂N₂O₃S Molecular Weight: 592,87 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD03453608 CAS-Nummer: 157134-53-7 |
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