Organic reference standard, Fast Green FCF, analytical standard, Supelco®
Lieferant: Merck
Fast Green FCF is a synthetic organic dye belongs to the triphenylmethane group. It has wide range of applications in food and beverage industry. It can also be used in research for staining of proteins and identification of extracellular recording or injection sites by electrophysiological experiments.
It is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast green staining is linear over a wider range of protein concentrations than brilliant blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity.
The gel can then be stained for 2 hours with 0,1% fast green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approximately 30% that with brilliant blue R.
Verpackung: Bottomless glass bottle. Contents are inside inserted fused cone.
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