JC-1 chloride salt Mitochondrien-Färbemittel, geeignet für Fluoreszenz

Lieferant: Biotium

Synonyme: 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride)

70011
70011.EA 376 EUR
70011.
JC-1 chloride salt Mitochondrien-Färbemittel, geeignet für Fluoreszenz
JC-1 chloride salt

JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.


  • Cellular localization: Mitochondria
  • For live or fixed cells: For live/intact cells
  • Assay type/options: No-wash staining, Real-time imaging
  • Cell permeability: Membrane permeant
  • Apoptosis/viability marker: Mitochondrial potential
  • Potential dependence: Mitochondrial potential-dependent
  • Indicator type: Ratiometric
  • Colors: Green/Red
  • Excitation/Emission: 510/527 nm (monomer); 585/590 nm (aggregate)
  • Red solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution


JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (λ Em = 527 nm),while the J-aggregates have red fluorescence (λ Em = 590nm). Therefore, it is possible to use the fluorescence ratio technique to study mitochondrial membrane potentials. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green. It has also been used in high throughput drug screening applications.


JC-1, iodide (#70014) is the original salt form of the dye. Biotium developed JC-1 chloride salt for applications where iodide ion is not desired. JC-1 chloride may also have the advantage of being slightly more water soluble than the iodide salt.

Bestellen

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Mehr About VWR