8-Aminonaphthalen-1,3,6-trisulfonsäure Dinatriumsalz fluoreszierender Farbstoff
Lieferant: Biotium
Synonyme:
8-Amino-1,3,6-naphthalenetrisulphonic acid disodium salt, Koch acid disodium salt, Amino H acid disodium salt, 1-Naphthylamine-3,6,8-trisulphonic acid disodium salt, Disodium 8-Amino-1,3,6-naphthalenetrisulphonate, ANTS, Disodium-8-amino-1,3,6-naphthalenetrisulphonate
ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers.
- λEx/λEm = 353/520 nm
- ε= 65,000
- Light yellow solid soluble in water and DMSO
- Store at 4°C and protect from light, especially in solution
ANTS has also been used together with the fluorescent quencher DPX (80012) for the studies of membrane fusion or permeability. The mixture of ANTS-DPX is minimally fluorescent initially, but becomes increasingly more fluorescent upon dilution (i.e., membrane fusion or leakage).
Formel:
C₁₀H₉NO₉S₃ Molecular Weight: 427,32 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00070572 CAS-Nummer: 5398-34-5 |
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