5(6)-Carboxyfluorescein diacetate NHS ester [CFSE (5(6)-CFDA SE)] ≥98% (durch TLC) fluoreszierender pH-Indikator
Lieferant: Biotium
Synonyme:
CFDA-SE, CFSE, Carboxyfluorescein diacetate succinimidyl ester, 5(6)-CFDA N-succinmidyl ester, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, 5(6)-CFDA SE
5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling.
- λEx/λEm (of hydrolysis product at pH 7) = 495/519 nm
- pKa 6.5
- Cell permeability: Membrane permeant
- Colors: Green
- White solid soluble in DMSO or DMF
- Store at -20°C and protect from light
The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation. Also see our CFDA SE Cell Proliferation Assay Kit (catalog no. 30050).
Formel:
C₂₉H₁₉NO₁₁ Molecular Weight: 557,467 g/mol Lagertemperatur: Gefrierschrank |
MDL:
MFCD00037466 CAS-Nummer: 150347-59-4 |
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