Arginase I (ARG1) Inhibitor Screening Kit (Colorimetric)
Lieferant: Abcam
The most sensitive kit to evaluate potential human Arginase I inhibitors.
- Simple and quick
- Used to identify and characterize ARG1 inhibitors in a high-throughput format
Arginase (EC 3.5.3.1) is a manganese-containing enzyme which catalyzes the conversion of arginine into urea and ornithine, which is the final reaction in the urea cycle. Arginase I (ARG1) is the liver isoform of arginase. Recent studies showed that ARG1 expression by mature myeloid cells in tumor environment as demonstrated in a 3LL murine lung carcinoma model causes L-Arginine depletion by tumor-associated myeloid cells (TAMC).
L-arginine depletion suppresses immune-response against tumor cells due to inhibition to T-cell proliferation. In addition, the depletion of arginine increases the reactive nitrogen species (NOS) and reactive oxygen species (ROS), which, in consequence, induce T-cell apoptosis and supports antigenic cell proliferation.
Arginase I (ARG1) Inhibitor Screening Kit is designed for screening ARG1 inhibitors. Two substituted-2-amino-6-hexanoic acids have been studied as arginase inhibitor and in this kit, Amino-2-Borono-6-Hexanoic Acid (ABH) is provided as a positive control. The ARG1 activity is monitored by the increase in absorbance readings (OD 450 nm), while potential inhibitors will cause a decrease of absorbance.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...