Oct4 transcription factor assay kit

Lieferant: Cayman Chemical

601080-96
CAYM601080-96EA 654 EUR
CAYM601080-96
Oct4 transcription factor assay kit
Tests Zelltests

96-Well assay for measurement of Oct4.


  • A sensitive, non-radioactive method of detecting Oct4 from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
  • Nuclear extraction kit is available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates


Octamer-binding transcription factor 4 (Oct4) is an essential regulator of embryonic stem cell pluripotency and used as a marker for undifferentiated cells. It also plays a role in gene expression, DNA repair and/or replication, signal transduction and tumorigenesis. Cayman’s Oct4 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor-DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the Oct4 response element is immobilized onto the wells of a 96-well plate. Oct4, contained in a nuclear extract, binds specifically to the Oct4 response element. Oct4 is detected by the addition of a specific primary antibody directed against Oct4. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.


Lieferumfang: kit contains assay buffer, reagent A, positive control, binding buffer, primary antibody, wash buffer concentrate, polysorbate 20, competitor dsDNA, HRP conjugate, plate and cover sheet, developing and stop solution.

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