NUCLEAR-ID® green chromatin condensation detection kit
Lieferant: ENZO LIFE SCIENCES
This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.A highly permeable green-emitting dye for enhanced detection of apoptosis-induced chromatin condensation.
- Dye excitable with standard 488 nm laser
- Intercalating dye with superior permeability with any live cell line
- Easy no-wash mix and read protocol
- Eliminates need for specialized 350 nm UV laser required for Hoechst dyes and reduces chances for channel interference
- No interference from small molecule fluorescence or cell autofluorescence
The Nuclear-ID® Green Chromatin Condensation Detection Kit detects nuclear condensation using a DNA intercalating dye that brightly stains the condensed chromatin of apoptotic cells, but only dimly stains the uncondensed chromatin of healthy living cells.
The cell-permeable dye used in this application is an aromatic, planar cationic structure that inserts between stacked base pairs on the DNA duplex, providing an environmentally-dependent fluorescence enhancement of the dye molecules and large increases in fluorescence signal relative to the free dye in solution. Since the signal enhancement provides a proportional response, direct quantitation of DNA is possible.
Further signal increase is observed upon DNA condensation during apoptosis. Considering the general mutagenic effect of nucleic acid-binding dyes, careful storage and handling of this dye is recommended.
Lieferumfang: Kit contains Nuclear-ID™ Green Cell Cycle Detection Reagent, 100 µl Apoptosis inducer (Staurosporine), 50 nmoles 10X Assay Buffer, 30 ml.
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