Nick Translation DNA Labelling System 2.0
Lieferant: ENZO LIFE SCIENCES
The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.
- Ready-to-use Nick Translation Enzyme Mix minimises pipetting error
- Short labelling time – only 1 hour
- Validated with fluorophore, biotin, and digoxigenin labelled dUTPs
The Nick Translation DNA labelling system 2.0 can accommodate a wide range of fluorophore-labelled, biotin-labeled, and digoxigenin-labelled nucleotides. In addition to choice of label, the kit design allows the user to optimise incorporation and product size by adjusting the ratio of labelled-dUTP to dTTP. The ready-to-use NT Enzyme Mix is user friendly and minimises error from pipetting.
Probes labelled by Nick translation can be used in many different hybridisation techniques including: in situ hybridisation (ISH), fluorescent in situ hybridisation (FISH), screening gene banks by colony or plaque hybridisation, DNA or RNA transfer hybridisation, and reassociation kinetic studies.
Lieferumfang: Contents: NT Enzyme mix 250 µl; Reaction buffer 250 µl; dNTP Mix (dATP, dGTP, dCTP) 250 µl; dTTP 180 µl; Stop buffer 250 µl; Nuclease-free water 5 ml.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...