Taq DNA Polymerase (native, with BSA)
Lieferant: Thermo Fisher Scientific
Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli. Native Taq DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.
- Thermostable with half life more than 40 minutes at 95 °C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers, including 10X Taq buffer with (NH₄)₂SO₄, which allows PCR at a wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labelled nucleotides)
Lieferumfang: Supplied with 10X Taq buffer with KCL, 10X Taq buffer with (NH₄)₂SO₄, 25 mM MgCl₂ and 20 mg/ml BSA.
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