Harnstoff ≥99%, weißes Pulver, ultrarein
Lieferant: MP Biomedicals
Synonyme:
Urea, Aminoameisensäureamid, Carbamid, Carbonyldiamid
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.
Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.
One gram dissolves in 1 ml water, 10 ml 95% ethanol (100 mg/ml), 1 ml boiling 95% ethanol, 20 ml absolute ethanol (50 mg/ml), 6 ml methanol (166 mg/ml), 2 ml glycerol (500 mg/ml); soluble in concentrated hydrochloric acid; almost insoluble in chloroform, ether. Solutions can be treated to remove isocyanic acid. Acidification (100 mM HCl) can be used to drive the equilibrium to favor urea over the isocyanic acid, or a urea solution can be bulk deionised using an ion exchange resin.
Bitte beachten Solutions of urea develop a significant concentration of reactive cyanate ions on standing. MP recommends making solutions fresh for each use.
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Formel:
NH₂CONH₂ Schmelzpunkt: 130…140 °C |
MDL:
MFCD00008022 CAS-Nummer: 57-13-6 |
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