Monomeric avidin agarose kit, Pierce™
Lieferant: Thermo Fisher Scientific
Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.
- Purifies biotinylated products using mild elution conditions
- Monomeric avidin resin can be regenerated and reused at least 10 times
- Retains biotin-binding specificity and low non specific binding of native avidin
- Bind samples in a variety of physiological buffers
- Binding capacity > than 1,2 mg biotinylated BSA/ml resin
During monomeric avidin immobilisation, polymeric forms of avidin with strong binding characteristics also are immobilized. These high affinity biotin-binding sites must first be blocked with a biotin-containing buffer. Biotin molecules are eluted from monomers with a glycine solution revealing only the reversible binding sites. The biotinylated molecule of interest may then be applied to the support for purification and then eluted by ligand competition using a biotin-containing solution.
Lieferumfang: Kit includes Pierce™ Monomeric Avidin column, 2 ml (1 column), PBS pack, makes 500 ml (1 pack), Biotin Blocking and Elution Buffer (200 ml), Regeneration Buffer (250 ml), and column accessories.
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