Boronic acid resin
Lieferant: Thermo Fisher Scientific
Pierce™ Boronic acid resin enables ribonucleotide and oligonucleotide RNA isolation.
- Support: Beaded polyacrylamide (1800 MW exclusion limit)
- Spacer: m-aminophenyl group
- Loading: 100 µMol boronate per ml of settled resin
- Capacity: at least 99% binding and recovery of 110 µMol AMP per ml of settled resin
- Format: 50% resin slurry in 0,05% sodium azide
The immobilised boronic acid is an easy to use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. Boronic acid affinity chromatography has been used to isolate ribonucleoside, to purify nucleosidyl peptide, separate ribonucleosidases in tissue extracts, separate RNA and oligoribonucleotide, and determine of the amount of non-enzymatic glycosylation present in peripheral nerve from diabetic and control rats and dogs.
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