DTBP (Dimethyl 3,3′-dithiopropionimidate dihydrochloride), Pierce™
Lieferant: Thermo Fisher Scientific
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Achtung
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Synonyme:
Wang/Richard Reagenz, DTBP
Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
- Reactive groups: imidoester (both ends)
- Reactive towards: amino groups (primary amines)
- Imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
- Cleavable by reduction of spacer arm disulfide bond with DTT, TCEP or other reducing agents
- Compare to DMS (non-cleavable)
- Water-soluble
Dimethyl dithiobispropionimidate (DTBP) is a homobifunctional, cleavable and membrane permeable crosslinker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₈H₁₆N₂O₂S₂·2HCl Molecular Weight: 309,28 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00043245 CAS-Nummer: 38285-78-8 |
Spezifikation Testergebnisse
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Homobifunctional |
Reactive Moiety | Imidoester |
Spacer | Cleavable, Mid-Length |
Form | Powder |
Quantity | 1 g |
Type | Crosslinker |
Solubility | Water |
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