EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
Lieferant: Thermo Fisher Scientific
Synonyme:
Bis(2,5-dioxopyrrolidin-1-yl) O,O'-ethane-1,2-diyl disuccinate, EGS, Ethylene glycol disuccinate di(N-succinimidyl) ester, Ethylene glycol bis(succinimidyl succinate), Di(N-succinimidyl) ethylene glycol disuccinate
21565
PIER21565EA
258
EUR
PIER21565
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)]
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
- Membrane-permeable, allowing for intracellular crosslinking
- Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₈H₂₀N₂O₁₂ Molecular Weight: 456,36 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00049057 CAS-Nummer: 70539-42-3 |
Spezifikation Testergebnisse
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Homobifunctional |
Reactive Moiety | NHS Ester |
Spacer | Cleavable, Long |
Form | Powder |
Quantity | 1 g |
Type | Crosslinker |
Solubility | DMF, DMSO |
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