LC-SPDP (Succinimidyl 6-[3-(2-pyridylthio)propionamidohexanoate), Pierce™
Lieferant: Thermo Fisher Scientific
Synonyme:
LC SPDP
Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
- Reactive groups: NHS ester and pyridyldithiol
- Reactive towards: amino and sulfhydryl groups
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Water-insoluble (dissolve first in DMF or DMSO)
- SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cells
- Compare to other varieties of SPDP-type reagents, including pegylated forms
We offer three analogs of SPDP: the standard version (SPDP), a derivative with a longer spacer arm (LC-SPDP), and a sulfonated water-soluble variety (Sulfo-LC-SPDP). SPDP (N-Succinimidyl 3-(2-pyridyldithio)-propionate) and LC-SPDP (Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate) are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₈H₂₃N₃O₅S₂ Molecular Weight: 425,522 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00083163 CAS-Nummer: 158913-22-5 |
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