EMCH.TFA (E-Maleimidocaproic acid hydrazine trifluoroacetic acid salt) ≥90% (durch NMR), Pierce™
Lieferant: Thermo Fisher Scientific
Synonyme:
EMCH
Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
- Reactive groups: maleimide and hydrazide
- Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
- Mid-length (11.8Å), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
- Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
- Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
- Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
- Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
3,3'-N-[ε-Maleimidocaproic acid] hydrazide, trifluoroacetic acid salt (EMCH) is a water-soluble, heterobifunctional, membrane permeable crosslinker. It contains a sulfhydryl reactive maleimide group and carbonyl reactive hydrazide group at each end of a 6-carbon spacer arm. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thiol ether bonds, along with release of the maleimide leaving group. Proteins with cysteine residues not involved in disulfide bond formation are targets for maleimide reactive groups. Carbonyl groups, whilst not found naturally on proteins can be formed by ring sugar reduction to form aldehydes which react with hydrazides at pH5.5-7.5.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₀H₁₅N₃O₃·C₂HF₃O₂ Molecular Weight: 339,27 g/mol Schmelzpunkt: 99…110°C Lagertemperatur: Kühlschrank |
MDL:
MFCD07357647 CAS-Nummer: 151038-94-7 |
Spezifikation Testergebnisse
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Reactive Moiety | Amine, Hydrazide, Maleimide |
Spacer | Mid-Length |
Form | Solid |
Quantity | 50 mg |
Type | Crosslinker |
Solubility | Water |
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