Sulpho-EMCS (N-(ε-maleimidocaproyloxy) sulphosuccinimide ester), Pierce™

Lieferant: Thermo Fisher Scientific

Synonyme: Sulfo EMCS

22307
PIER22307EA 419 EUR
PIER22307
Sulpho-EMCS (N-(ε-maleimidocaproyloxy) sulphosuccinimide ester), Pierce™
Sulpho-EMCS (N-(ε-maleimidocaproyloxy) sulphosuccinimide ester)

Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).


  • Reactive groups: sulfo-NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
  • Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
  • Non-cleavable
  • Water-soluble (compare to EMCS)
  • Aliphatic spacer offers low potential for eliciting an immune response


Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ∼10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.


Bitte beachten For Research Use Only. Not for use in diagnostic procedures.

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Spezifikation Testergebnisse

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Maleimide, Sulfo-NHS Ester
Spacer Short
Form Powder
Quantity 50 mg
Type Crosslinker
Solubility Water

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