LC-SMCC (N-Succinimidyl 6-[[4-(N-Maleimidomethyl)cyclohexyl]carboxamido]hexanoate), Pierce™
Lieferant: Thermo Fisher Scientific
Synonyme:
LC SMCC
Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).
- Reactive groups: NHS ester and maleimide
- Reactive toward: amino and sulfhydryl groups
- Long-chain variety of SMCC
- High purity, crystalline reagent can be used to create high-purity maleimide-activated derivatives
- Non-cleavable
- Water-insoluble (dissolve first in DMF or DMSO)
- Cyclohexane bridge confers added stability to the maleimide group making LC-SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.
LC-SMCC is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of LC-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₂₂H₂₉N₃O₇ Molecular Weight: 447,49 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD01863459 CAS-Nummer: 125559-00-4 |
Spezifikation Testergebnisse
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Reactive Moiety | Maleimide, NHS Ester |
Spacer | Long |
Form | Solid |
Quantity | 50 mg |
Type | Crosslinker |
Solubility | DMF, DMSO |
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