SPB (Succinimidyl-[4-(psoralen-8-yloxy)]butyrate), Pierce™
Lieferant: Thermo Fisher Scientific
Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.
- Reactive groups: NHS ester and psoralen
- Reactive toward: amino groups and thymine
- DNA intercalating agent and nucleic acid probe
- Reagent for photo-immobilization to microplates
Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ∼10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₉H₁₅NO₈ Molecular Weight: 385,32 g/mol Schmelzpunkt: 177…178°C Lagertemperatur: Kühlschrank |
MDL:
MFCD29041827 CAS-Nummer: 858128-57-1 |
Spezifikation Testergebnisse
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Reactive Moiety | Psoralen, NHS Ester |
Spacer | Short |
Form | Solid |
Quantity | 50 mg |
Type | Crosslinker |
Solubility | DMF, DMSO |
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