LC-SDA (NHS-LC-Diazirine) (succinimidyl 6-(4,4′-azipentanamido)hexanoate), Pierce™

Lieferant: Thermo Fisher Scientific

Synonyme: LC-SDA

26168
PIER26168EA 486 EUR
PIER26168
LC-SDA (NHS-LC-Diazirine) (succinimidyl 6-(4,4′-azipentanamido)hexanoate), Pierce™
LC-SDA (NHS-LC-Diazirine) (succinimidyl 6-(4,4′-azipentanamido)hexanoate)

Thermo Scientific Pierce LC-SDA (NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.


  • Membrane-permeable—suitable for in vivo intracellular protein crosslinking
  • Heterobifunctional—NHS ester group reacts with primary amines at pH 7 to 9 to form covalent amide bonds; diazirine (azipentanoate) group reacts efficiently with any amino acid side chain or peptide backbone upon activation with long-wave UV light (330-370 nm)
  • Controllable—two-step chemical crosslinking is activated using common laboratory UV lamps
  • Easy to use—these crosslinkers are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark
  • Better than aryl azides—the diazirine photoreactive group has better photostability in normal light than phenyl azide groups of traditional photoreactive crosslinkers, yet the diazirine group is more efficiently activated by long-wave UV light


Succinimidyl-diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions, and these SDA reagents extend the efficiency and range of interactions that can be explored by this approach.


Bitte beachten For Research Use Only. Not for use in diagnostic procedures.

Bestellen

Spezifikation Testergebnisse

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety NHS Ester, Diazirine
Spacer Long
Form Solid
Quantity 50 mg
Type Crosslinker
Solubility DMF, DMSO
Spacer Arm 12.5 Å

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Mehr About VWR