Pierce™ screw cap spin columns are convenient tools for manipulating small volumes of affinity supports (20 to 400 µl) for protein purification.
- Adaptable - included Luer-Lok adaptors allow these columns to be used for syringe-based purifications
- Column volume: 900 µl
- Resin volume: 20 to 400 µl
- Filter type: PE filter, ~10 µm pore size
- Cap: O-ring screw-top caps
- Small and large frit options are included for different sample sizes
Columns can be placed in 1,5 ml or 2,0 ml microcentrifuge tubes or used with a Luer-lok adapter for processing samples with a syringe. When using a syringe, sample size and wash volumes are only limited by the volume capacity of the syringe. For small volumes of resin, use columns with only the small, pre-inserted frit. For applications requiring more than 100 µl of resins, the large frit may be used at either the top or bottom of the resin bed. Resins may be prepared for repeated use when the resin is sandwiched between the small and large frit.
Applications include affinity purification/affinity chromatography, immunodepletion, immunoprecipitation (IP), and co-immunoprecipitation (co-IP).
Format: Microcentrifuge, spin column
Column type: Empty column
