Sulpho-SANPAH (Sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), No-Weigh™ Format, Pierce™
Lieferant: Thermo Fisher Scientific
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Achtung
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Synonyme:
Sulfo SANPAH
Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
- Reactive groups: sulfo-NHS ester and nitrophenyl azide
- Reactive towards: amino groups and any nucleophile
- Non-cleavable
- N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
- Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
- Water soluble; non-cleavable
NHS esters react efficiently with primary amino groups (-NH2) in pH 7–9 buffers to form stable amide bonds. The reaction results in the release of sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines). Sulfo-SANPAH, supplied as a sodium salt, is useful for cell-surface protein crosslinking and possesses charged groups for water solubility to a concentration of 10 mM.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₆H₁₇O₉N₆NaS Molecular Weight: 492,40 g/mol Lagertemperatur: Gefrierschrank |
CAS-Nummer:
102568-43-4 |
Spezifikation Testergebnisse
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Reactive Moiety | Sulfo-NHS Ester, Aryl Azide |
Spacer | Long |
Form | Solid |
Quantity | 50 mg |
Type | Crosslinker |
Solubility | Water |
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