Natrium-1-hydroxy-2,5-dioxo-3-pyrrolidinsulfonat, No-Weigh™ Format, Pierce™
Lieferant: Thermo Fisher Scientific
Synonyme:
N-Hydroxy sulphosuccinimide sodium salt, Hydroxy-2,5-dioxopyrrolidine-3-sulphonic acid sodium salt, N-Hydroxysulphosuccinimide sodium salt, Sulfo NHS, Sulpho-N-Hydroxysuccinimide
Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
- Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
- Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule
- Sulfo-NHS derivatives are usually directly water-soluble (can be added directly to physiologic buffers) and membrane-impermable (can be used for cell surface labeling)
- High-purity, crystalline Sulfo-NHS can be used to create high-quality activated derivatives
- Purity—Greater than 95% by quantitative NMR (the highest standard for crosslinker purity); average lot purity is greater than 99%
Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₄H₄NNaO₆S Molecular Weight: 217,13 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00043100 CAS-Nummer: 106627-54-7 |
Spezifikation Testergebnisse
Spacer Arm Length | Short |
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