Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is a two-component, high precision, detergent-compatible assay optimised to measure (at 480 nm) total protein concentration compared to a protein standard curve of known concentrations. The Pierce™ Rapid Gold BCA Protein Assay uses the same copper-chelating technology as the well-known traditional Pierce™ BCA Protein Assay and provides comparable accuracy but with a 5 minute, room temperature incubation and measured at 480 nm. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results.
- Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (480 nm)
- Excellent uniformity – exhibits similar protein-to-protein variation as traditional BCA Protein Assay, and less protein-to-protein variation than dye-binding protein assay methods
- Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
- Fast time to results – significant improvement over traditional BCA Assay with a 5 minute, room temperature reaction
- High linearity – linear working range for BSA equals 20 to 2000 µg/mL
- Optimal absorbance on standard spectrophotometers – reaction produces a colour change to an intense orange-gold that results in a strong linear response at 480 nm
Applications:
Studying protein:protein interactions
Measuring column fractions after affinity chromatography
Estimating percent recovery of membrane proteins from cell extracts
High-throughput screening of fusion proteins
