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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-1B1301-KIT

VWR® Protein Precipitation Kit

Artikel-Nr: (1B1301-KIT)
Lieferant: VWR Chemicals
Beschreibung: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
VE: 1 * 1 KIT
Image Unavailable-D8284-5G

Bicinchoninic acid disodium salt hydrate

Lieferant: SIGMA ALDRICH MICROSCOPY
Beschreibung: Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Image Unavailable-CAYM10005020-96

Protein carbonyl colorimetric assay kit

Artikel-Nr: (CAYM10005020-96)
Lieferant: Cayman Chemical
Beschreibung: The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
VE: 1 * 96 Tests
Product Image-786-892

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Lieferant: G-Biosciences
Beschreibung: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Product Image-786-604

Protein assay kits, RED 660™

Lieferant: G-Biosciences
Beschreibung: RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Image Unavailable-33021-1

Thermal shift protein stability kits, GloMelt™

Lieferant: Biotium
Beschreibung: Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

Sicherheitsdatenblätter

Image Unavailable-TNB-1213-L150

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Artikel-Nr: (TNB-1213-L150)
Lieferant: Tonbo Biosciences
Beschreibung: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
VE: 1 * 150 mL
Product Image-CAYM700480-96

Glycogen Assay Kit

Artikel-Nr: (CAYM700480-96)
Lieferant: Cayman Chemical
Beschreibung: A simple and sensitive tool for assaying glycogen from tissue.
VE: 1 * 1 ST
Product Image-ROCKMB-061-0100

Conjugate stabilisers

Lieferant: Rockland Immunochemicals
Beschreibung: Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.

Image Unavailable-ENZOENZ51029K100

eFluxx-ID® multidrug resistance assay kit

Lieferant: ENZO LIFE SCIENCES
Beschreibung: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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Product Image-ENZOENZ51023KP050

PROTEOSTAT® protein aggregation assay

Artikel-Nr: (ENZOENZ51023KP050)
Lieferant: ENZO LIFE SCIENCES
Beschreibung: PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
VE: 1 * 50 Tests

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Product Image-786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Artikel-Nr: (786-005)
Lieferant: G-Biosciences
Beschreibung: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
VE: 1 * 1 KIT
Image Unavailable-ENZOBMLUW1000A0001

SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Artikel-Nr: (ENZOBMLUW1000A0001)
Lieferant: ENZO LIFE SCIENCES
Beschreibung: The only commercially available kit for isolation and enrichment of SUMOylated proteins.
VE: 1 * 10 Tests

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Product Image-GENO786-895

Micro Bicinchoninic Acid (BCA) protein assay

Lieferant: G-Biosciences
Beschreibung: The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

Product Image-786-571

Bicinchoninic acid (BCA) protein assay

Lieferant: G-Biosciences
Beschreibung: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Zertifikate

Product Image-CAYM700510-96

L-Lactate Assay Kit

Artikel-Nr: (CAYM700510-96)
Lieferant: Cayman Chemical
Beschreibung: A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
VE: 1 * 1 ST
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Lager für diesen Artikel ist begrenzt, kann aber in einem Lagerhaus in Ihrer Nähe zur Verfügung. Bitte stellen Sie sicher, dass Sie in sind angemeldet auf dieser Seite, so dass verfügbare Bestand angezeigt werden können. Wenn das call noch angezeigt wird und Sie Hilfe benötigen, rufen Sie uns an 1-800-932 - 5000.
Dieses Produkt kann nur an eine Lieferadresse versandt werden die über die entsprechende Lizenzen verfügt. Für weitere Hilfe bitte kontaktieren Sie Ihr VWR Vertriebszentrum.
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