BCIP/TNBT alkalines Phosphatase-Substrat
Lieferant: Rockland Immunochemicals
Synonyme:
TNBT (tetra nitro-blue tetrazolium chloride), BCIP (5-bromo-4-chloro-3-indolylphosphate toluidine salt)
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for WB, IHC (immunohistochemistry), and in situ hybridisation.
Storage Condition: Store container at 4° C prior to opening. Protect BCIP/TNBT Membrane Alkaline Phosphatase Substrate from moisture and light.
Spezifikation Testergebnisse
pH | 9.28 +/- 0.1 |
QC Raw Material | PASS |
Absorbance check of final product | PASS |
Performance Data per Immunoblot | PASS |
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